What is Brucellosis?
Brucellosis is an important venereal disease in many species. It is caused by infection with a bacteria from the genus, Brucella. It does not usually come up in pet ownership because most pet dogs are not used for breeding. Once someone has decided to breed their dog, though, it behooves them to know all about this disease, particularly since it can be transmitted to humans. If you are planning to breed your dog, test your dog and insist that the owner of your dog’s mate produce results of a recent test for your inspection prior to breeding. All actively bred studs should be tested every 6 months.
Which Type of Brucella?
There are six species of Brucella. The most common species infecting the dog is Brucella canis, however dogs can certainly become infected with Brucella abortus (from cattle), Brucella melitensis (from goats) and Brucella suis (from pigs) if they are allowed to drink contaminated milk or eat leftover birth membranes, contaminated meat, or aborted young. (The other two species are Brucella ovis, which affects only rams, and Brucella neotomae, which affects desert mice.) Luckily for humans, Brucella canis causes much less serious disease in humans than do the livestock Brucellae, but the health department still considers any Brucella infection reportable.
How Dogs Get Infected:
Dogs like to stick their noses in all sorts of nasty places. They also chew up all sorts of disgusting things. Brucellosis can be contracted sexually, but it can also be contracted by inhalation (sniffing contaminated urine or fetal membranes), through the eyes, or orally (licking contaminated urine or urogenital secretions, or chewing up fetal membranes). Urine and saliva from an infected dog are not nearly as contagious as urogenital secretions. It is when breeding and/or whelping is in the picture that the transmission risk becomes very high.
When a female dog aborts a pregnancy because of a Brucella infection, she continues to secrete fluids packed with Brucella bacteria for 4 to 6 weeks.
What Happens After Infection?
The organism requires an average of three weeks to become evident in the bloodstream. After that they localize in the reproductive or urinary tract, and either continuously or periodically seed the bloodstream from there. Lymph nodes can enlarge, and possibly the spleen or liver can become inflamed, but generally the infected adult dog does not seem sick. Chronic disease from long-term immune stimulation can result. This can include:
- Discospondylitis (inflammation of a disc in the spine)
- Uveitis (deep eye inflammation)
- Multiple joint arthritis
- Glomerulonephritis (kidney inflammation and protein loss)
Most of the time, the only sign is aborted pregnancy between the 45th and 59th day of pregnancy (relatively late in the pregnancy). Classically, the aborted pups appear to have died at least several days prior to abortion, as they do not look freshly dead. Abortion does not always appear in this most common form, though. Sometimes the pregnancy is lost much earlier and is deemed to represent infertility. Sometimes puppies are still born. Sometimes they are born live and infected.
Direct culture of the organism from a dead puppy, from an infected dog’s blood, or from a secretion is confirmatory but the organism is difficult to isolate in this way. This means we usually depend on immunologic tests. Which test is selected depends on what the test is being used for.
Screening Before Breeding:
The RSAT (Rapid Slide Agglutination Test) is a screening test that can readily identify negative dogs. That is, if the test comes out negative, the dog can be considered negative. If the test comes out positive, further testing is needed. Up to 60% false positives occur. A test kit is available for use inside the veterinary hospital and some facilities can perform this test while you wait.
The IFA (Immunofluorescent Antibody) test is a similar screening test but it must be sent to the reference laboratory. The same guidelines apply: negative means negative, positive means do further tests.
Further Testing For RSAT or IFA Positive Dogs:
There are two tests that fit in this category. The most specific test (meaning the most trustworthy positive value) is the AGID (Agar Gel Immunodiffusion) test. A version of the test called the CPAGID (named for the bacterial protein it detects) is the most accurate of all.
Another test is called a TAT (Tube Agglutination Test). It looks for antibodies against Brucella canis. Antibiotic treatment with tetracyclines can drop antibody levels low enough for the TAT to be negative but this does not necessarily indicate that the infection has cleared. At this time the CPAGID is favored over TAT.
- If a dog is to travel to Australia, a TAT test is required as part of the travel documentation.
- If a dog is to travel to New Zealand, an AGID test is required as part of the travel documentation.
First of all, the infected dog must be removed from the breeding program (spayed, neutered or euthanized) and isolated from any animals to be used for breeding. The dog can remain as a pet, but probably should not be sold due to potential health risks to potential buyers. For pet dogs, a course of antibiotics can be given, but since this bacterium is so good at hiding inside the host’s cells, one can never assume it is ever truly gone. Some people keep their dogs on tetracycline for life. Dogs do recover from this infection without treatment, but it can take up to five years. Naturally-recovered dogs cannot be reinfected; antibiotic-treated dogs can be reinfected. Human health must be considered in the decision to keep and treat an infected dog.
Keeping Brucella Out of the Kennel:
A new dog for a breeding kennel should be isolated for one month. Two Brucella tests one month apart should be adequate to confirm negativity (it takes about three weeks from infection for tests to turn positive). If an infection is detected in a kennel, the entire kennel population should be evaluated.
References: Portions of this handout were taken from this source: